RAPPAPORT-VASSILIADIS (RV) BROTH 500g -Oxoid CM0669

RAPPAPORT-VASSILIADIS (RV) ENRICHMENT BROTH
Code: CM0669
THIS MEDIUM IS VERY HYGROSCOPIC AND MUST BE PROTECTED FROM MOISTURE.
The quantities given for the formula as classically described made 1110ml of medium. They have been published this way in the Oxoid literature to coincide with the scientific literature.
The directions for reconstituting Oxoid Rappaport-Vassiliadis (RV) Enrichment Broth follow usual Oxoid practice and specify the weight needed for 1 litre of medium.
Directions
Add 30g (the equivalent weight of dehydrated medium per litre) to 1 litre of distilled water. Heat gently until dissolved completely. Dispense 10ml volumes into screw-capped bottles or tubes and sterilise by autoclaving at 115°C for 15 minutes.
Description
Rappaport-Vassiliadis (RV) Enrichment Broth is based on the formulation described by van Schothorst and Renaud1 and is recommended as the selective enrichment medium when isolating Salmonella species from food and environmental specimens. It can also be used to isolate Salmonella from human faeces without pre-enrichment but the inoculum must be small. The original formulation described by Rappaport et al2 was specifically developed to exploit the four characteristics of Salmonella species when compared with other Enterobacteriaceae.
1. The ability to survive at relatively high osmotic pressures.
2. To multiply at relatively low pH values.
3. To be relatively more resistant to malachite green.
4. To have relatively less demanding nutritional requirements