OXOID RAPPAPORT VASSILIADIS (RVS) PEPTONE BROTH 500 GR - CM0866B

RAPPAPORT-VASSILIADIS SOYA PEPTONE BROTH (RVS BROTH)
Code: CM0866
Description
Rappaport-Vassiliadis Soya Peptone Broth (RSV Broth) is recommended as a selective enrichment medium for the isolation of salmonellae from food and environmental specimens. It shares with the original formulation1, the ability to exploit the full characteristics of Salmonella species when compared with other Enterobacteriaceae. These are:
1. The ability to survive at relatively high osmotic pressure.
2. To multiply at relatively low pH values.
3. To be relatively more resistant to malachite green.
4. To have relatively less demanding nutritional requirements.
RVS Broth is based on the revised formulation described by van Schothorst et al.2, and is recommended as the selective enrichment medium for the isolation of salmonellae from food and environmental specimens. It can also be used to isolate salmonellae from human faeces without the need for pre-enrichment. It is a modification of the Rappaport Vassiliadis (RV) Enrichment Broth described earlier by van Schothorst and Renauld3. The modifications to their earlier formula are:
1. The addition of di-potassium hydrogen phosphate to buffer the medium so that the pH is maintained during storage of the prepared broth.
2. Clarifying the optimum concentration of magnesium chloride 6H2O.
The two modifications are said to enhance the reliability of the enrichment broth1. Peterz et al.4 have also highlighted the importance of the concentration of magnesium chloride in the final medium.